Green Guardians: How Moss is Helping Monitor Chicago’s Air Pollution

Researchers used simple moss bags and portable X-ray technology to track urban heavy metal pollution, offering a cheap, effective tool for environmental monitoring.

Air pollution is an invisible threat in our urban environments, heavily impacting cardiovascular and respiratory systems and standing as the single largest environmental cause of disease and premature death globally. 

Often, monitoring the heavy metals and particulate matter that fill city air involves expensive equipment, heavy use of chemical reagents, and time-consuming laboratory preparation. However, researchers in Chicago have been exploring a much cheaper, greener alternative that might already be growing nearby: moss. The full research is available in the open-access, peer-reviewed journal BioRisk.

Because mosses lack true root systems, they absorb the vital nutrients they need to survive, along with any environmental contaminants, directly from the atmosphere, making them exceptionally effective living records of long-term air pollution.

Sphagnum moss
Sphagnum moss by andipandz via Canva.

To test the viability of this biological monitoring, the team systematically deployed small, one-gram mesh bags of carefully cleaned Sphagnum moss across various locations in the Chicago area.

The scientists strategically hung these “green guardians” in diverse environments, including an industrial metals recycling facility, a marshland corridor, a commuter railroad station, and a semi-natural reference area in Lake County. 

Moss biobags
A. Biobags attached to branches in a semi-natural reference area; B. Biobags taped to the exhaust of a Ford F-350 diesel truck. Credit to Campbell et al., 2026

In a direct test of vehicle emissions, they even taped several moss bags straight to the exhaust pipe of a diesel truck. After leaving the moss bags exposed to the urban elements for roughly three to four weeks, the team brought them back to the lab, crushed them into a homogeneous powder using liquid nitrogen, and analyzed them using portable X-ray fluorescence (pXRF) technology

The living monitors proved incredibly adept at capturing location-specific pollution footprints. Unsurprisingly, the moss placed near the metals recycling facility showed elevated levels across all the measured heavy metals, acting as a clear multi-metal emission source. The bags stationed near the commuter railroad exhibited significant spikes in iron, a likely consequence of the friction and wear on the steel rail infrastructure. 

Alarmingly, even the moss placed in the semi-natural Lake County area absorbed elevated concentrations of nickel, demonstrating that pollution from a major highway less than one kilometer away can travel far into a seemingly pristine environment. 

Ratio of pXRF counts for exposed samples to pXRF counts for unexposed samples for six metals. The horizontal line represents a ratio of 1, i.e., no net accumulation of that metal in the exposed samples. Error bars represent the standard deviation of the ratio of the measurements of the exposed samples to the unexposed samples. Credit to Campbell et al., 2026

Furthermore, the moss bags directly attached to the diesel truck showed a rapid accumulation of metals like iron, zinc, copper, titanium, and manganese within just three days, though a heavy Chicago rainstorm on the fourth day washed some of that accumulated particulate matter away. 

Ultimately, this exploratory study demonstrates that combining simple moss bags with portable X-ray technology can serve as a highly accurate, cost-effective alternative to conventional air monitoring. Since the preparation is cost-effective and the screening process is relatively quick, this methodology has the potential to make environmental science more affordable and accessible; therefore, this study warrants further research.

Moving forward, resource-limited communities that are disproportionately burdened by poor air quality could utilize these green guardians to document local contamination hotspots. By doing so, they can gather the vital data needed to fiercely advocate for environmental justice, targeted remediation, and healthier urban spaces for everyone.

Original source:

Campbell T, Gwilliam III GF, Evans NM, Peltz K, Simpson G, Dussubieux L, Erazo E, Guare K, Krechner A, Long E, Maari S, Oguike I, Rice K, Zinke A, Christian C, Qazi-Lampert A, Henkin JM, Kreider D, Maves C, Merrill G, Moy P, Rodriguez Y, Ryan Z, von Konrat M (2026) Green guardians: Mosses as potential low-cost biomonitors of air pollution in urban Chicago. BioRisk 24: 55-67. https://doi.org/10.3897/biorisk.24.180507

New DNA Barcode Reference Data For Freshwater Diatoms (Bacillariophyceae) From Sweden

New study in Metabarcoding and Metagenomics shares results from FRESHBAR, the first large-scale barcode reference for Nordic benthic freshwater diatoms.

Guest blog by Maria Kahlert

Diatom DNA metabarcoding holds great potential for biodiversity monitoring and ecological assessment, particularly within the EU Water Framework Directive (WFD) and the recently introduced EU Nature Restoration Law (NRL). However, several challenges remain, among which gaps in reference databases have been identified as a major obstacle, especially for understudied habitats and ecoregions.

  •  Ribbon-shaped chains representing colony formation of Eunotia implicata Nörpel, Lange-Bertalot & Alles
  • Stacked chain colonies of Eunotia incisa W.Smith ex W.Gregory

In this context, we present results from the national barcoding project (FRESHBAR), conducted between 2019 and 2023 and focusing on benthic freshwater diatoms of Sweden, a key organism group for both ecology and environmental assessment. The study was recently published in the open-access Metabarcoding and Metagenomics journal. A primary goal of the project was the publication and vouchering of all data, materials, and results to support further research. 

  • Pin-cushion-like colonies (tufts of upright cells sharing a common pad of mucilage at the base) of Eunotia cf. seminulum Nörpel-Schempp & Lange-Bertalot
  • Pin-cushion-like colonies of cells of the Eunotia flexuosa/ pseudoflexuosa/ latitaenia – group

The project established a total of 312 diatom cultures, with a focus on oligotrophic and acidic habitats. The cultures were sequenced for two barcodes (rbcL and 18SV4) and identified using light microscopy, while selected strains were additionally examined by scanning electron microscopy. All data, including sampling metadata, barcode sequences, images, and voucher material, were published in accordance with the FAIR principles, and a subset of cultures was archived in diatom culture collections.

Chain-forming colonies in bracelet form of the Eunotia flexuosa/ pseudoflexuosa/ latitaenia – group.
Chain-forming colonies in bracelet form of the Eunotia flexuosa/ pseudoflexuosa/ latitaenia – group. Credit to Maria Kahlert.

Nearly all strains were successfully sequenced, identifying 51 taxa across 17 genera. A notable highlight was the relatively high proportion of Eunotia taxa, a genus poorly represented in diatom databases yet frequently encountered in Swedish freshwaters. Beyond molecular and morphological data, we also captured images of colony formation and sexual reproduction stages from living cultures, information that is only rarely documented.

  • Sexual reproduction (auxospore formation) and ribbon-shaped chains representing colony formation of Eunotia sp.
  • Sexual reproduction (auxospore formation) and ribbon-shaped chains representing colony formation of E. myrmica Lange-Bertalot

FRESHBAR represents the first large-scale effort to generate barcode reference sequences for Nordic benthic freshwater diatoms. As all data are publicly available, we are confident that the added sequences and morphological information will contribute to more accurate species-level identification, help resolve taxonomic relationships within diatoms, and improve reference databases for environmental monitoring and research.

Original source:

Kahlert M, Mora D, Kusber W-H, Abarca N, Zimmermann J (2026) New DNA barcode reference data of freshwater diatoms (Bacillariophyceae) from Sweden: old acquaintances and new taxa. Metabarcoding and Metagenomics 10: e186778. https://doi.org/10.3897/mbmg.10.186778