dna sequencing

A New Class Of Fungi Named After The King Of Sweden

A newly discovered fungal species has been named after the King of Sweden to mark the 50th anniversary of his reign.

Originally published by Uppsala University

Researchers at Uppsala University have discovered a completely new fungal species, which they chose to name after the King of Sweden. The species has been given the Latin name Semicentenialea rex, which means fifty-year anniversary of the King. It is the first known representative of a new class of fungi that will be called Semicentenialomycetes.

The new fungus was isolated from roots in the ground in Jädraås in the province of Gästrikland, and represents a new lineage among what are primarily rust fungi. Rust fungi are a type of fungi that causes diseases in plants, usually by infecting the leaves. Based on DNA sequencing from soil samples, the researchers know that the class occurs in soil and roots from different ecosystems across almost the entire world, but rarely at high abundance.

the new fungi Semicentenialea rex — The images show *Semicentenialea rex* both as a colony and under the microscope. After eight weeks of growth, the fungus forms a small colony on the culture plate. The microscopic images reveal a network of hyphae with so-called clamp connections – structures that connect adjacent cells in the hyphae of basidiomycete fungi. To follow fungal development, the researchers stained the nuclei pink and the cell walls blue. Three types of swollen cells can be observed within the hyphae: basidium-like cells, teliospore- or probasidium-like cells, and intercalary swellings. These structures are examined in greater detail in the following figures. Credit to Anna Rosling.

There are probably at least two other species in the class, but they do not appear to be as common as Semicentenialea rex. Since it only seems to live deep down in the ground, we are curious about what life strategy it has. We are going to study how it affects the roots of plants in the soil and how it propagates.
says Anna Rosling, professor at Uppsala University and one of the researchers behind the study.

Several million species of fungi

phylogenetic tree of Semicentenialea rex — The researchers analysed hundreds of genes to determine where the newly discovered fungus *Semicentenialea rex* belongs in the fungal tree of life. The results show that *S. rex*, previously known as GS25, forms a distinct lineage within the *Pucciniomycotina*. To confirm its placement, genes from both closely related and more distantly related fungi were compared, including species from both the Basidiomycota and the Ascomycota. Credit to Anna Rosling.

The species diversity in kingdom Fungi is largely unexplored. Based on sequencing of environmental DNA, it is estimated that there are several million species of fungi across the globe. But because most live concealed in the soil, wood or insects for example, it has only been possible to describe and name a small proportion of them. Without names, it is difficult to communicate about these species, genera, classes and even phyla of hitherto unknown fungi.

It was during a study root fungal diversity, when the researchers were culturing fungi from pine tree roots collected from mineral soil at Ivantjärnheden field station near Jädraås that the researchers identified a new fungal species that turned out to represent a new class of fungi.

Exciting with a root-associated fungus

Veera Tuovinen-Nogerius and Anna Rosling, both of Uppsala University, have led an international team of researchers in the work of characterising the morphology of the new species and its phylogenetic placement. The results have now been published in the International Mycology Association’s journal IMA Fungus.

A new class is exciting enough but in this case, it’s especially exciting because we have a root-associated fungus that evolutionarily speaking sits among the rust fungi in Puccinomycotina. Rust fungi are obligate parasites that infect plants and cause diseases above ground.
says Anna

Suitably named after the King of Sweden

This figure presents additional structures that are likely to play a role in the fungal life cycle. Among them are intercalary swellings and teliospore-like cells, which contain spore-like structures with one or more nuclei. The researchers documented several stages of development, ranging from young swellings and spore formation to germination and the emergence of new hyphae. Some structures produce thick promycelium-like hyphae, while others release small yeast-like cells through budding. These observations suggest that *Semicentenialea rex* has a complex life cycle involving several different cell types and spore forms. This resembles rust fungi within the *Pucciniomycotina*, which are known for producing multiple types of spores. Credit to Anna Rosling/Uppsala University.

Finding names for new species is a pleasant challenge for all researchers doing this type of research. This particular one was named Semicentenialea rex to pay tribute to King Carl XVI Gustaf of Sweden when he celebrated 50 years on the throne in 2023.

During his reign, the King has worked tirelessly for biodiversity conservation and the sustainable use of our natural resources. What could be better than to have a previously undescribed fungus named after you.
says Anna Rosling, who in connection with the official celebration of the King’s golden jubilee presented a picture and a description of the species to him.

Now that the species has a new name, it will facilitate future communication and studies of the role of Semicentenialea rex in the ecosystem.

Original source:

Nogerius VT, Sánchez-García M, Kluting K, Heinonsalo J, Ryberg M, Toome M, Haridas S, Mondo S, LaButti K, Nolan M, Lipzen A, Koriabine M, Bauer D, Barry K, Grigoriev IV, Aime MC, Rosling A (2026) Another dark taxon comes to light: Semicentenialomycetes, a new class within the Pucciniomycotina (Basidiomycota), and its first described representative, Semicentenialea rex. IMA Fungus 17: e189848. https://doi.org/10.3897/imafungus.17.189848

Study on mysterious Amazon porcupine can help its protection

After 22 years of relative obscurity, this research uncovers vital information about its distribution, phylogenetics, and potential conservation threats.

Porcupines of the genus Coendou are arboreal, herbivorous, nocturnal rodents distributed in tropical and subtropical regions of the Americas. Most of what we currently know on them is restricted to species that occur near urban areas, and we still have a lot to learn about these fascinating animals.

Recently, a new study shed light on a very unknown neotropical porcupine species. Roosmalens’ dwarf porcupine (Coendou roosmalenorum) is the smallest porcupine species we know, with blackish monocolored bristles on the tail which confers a blackish color to it, but apart from its appearance, we didn’t know much about it until recently.

A preserved specimen of *Coendou roosmalenorum*.

“This species was described in 2001 and our paper is the first scientific report after this date, which means nothing was discovered about Roosmalen’s porcupine in a 22-year period,” says Fernando Heberson Menezes, the lead author of a study that was just published in the open-access journal ZooKeys.

“Before our research, we had only a morphological description of the species, with a little information about its distribution and natural history, and nothing about population ecology or conservation threats.”

Using DNA sequencing and exploring data on its occurrences, Fernando and his team were able to uncover new facts about the enigmatic animal.

Thanks to their study, we now know more about its distribution in the Madeira biogeographical province in the Amazon Forest. “With this information, we raised the hypothesis this species is endemic to Madeira Province, which is important for predicting where we can find this species and the possible threats affecting its population or its distribution,” says Fernando.

Distribution of *Caaporamys roosmalenorum* in Brazilian Amazonia. The new record (locality 1) is the southeastern most record for the species, from Mato Grosso state, Brazil. The darker gray area represents the Madeira Province sensu Morrone et al. (2022).

At the same time, they found Roosmalens’ dwarf porcupine at new locations in the Amazon rainforest, which suggests that its distribution in southern Amazonia is wider than suspected.

Their phylogenetic analysis – the study of the species’ evolutionary history and relationships with other species – confirmed that the species is a member of the subgenus Caaporamys . This is important, the researchers say, because the classification of the genus Coendou had been “historically chaotic” until the last few years.

The information in this study opens up numerous opportunities for further researching this species. “We can think of ways to answer very basic scientific questions such as ‘how does Roosmalen’s porcupine use space?’ or ‘what does it eat?’, some more advanced questions such as ‘how did it evolve?,’ or applied questions such as ‘what are the major threats for its conservation?,’ or ‘how can we use it as a model to know more about the health of the Amazon forest?’, says Fernando in conclusion.

Original source:

Menezes FH, Semedo TBF, Saldanha J, Garbino GST, Fernandes-Ferreira H, Cordeiro-Estrela P, da Costa IR (2023) Phylogenetic relationships, distribution, and conservation of Roosmalens’ dwarf porcupine, Coendou roosmalenorum Voss & da Silva, 2001 (Rodentia, Erethizontidae). ZooKeys 1179: 139-155. https://doi.org/10.3897/zookeys.1179.108766

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Newly established Bulgarian Barcode of Life to support biodiversity conservation in the country

As the latest national node to join the International Barcode of Life Consortium (iBOL), its main task is to coordinate, support, and promote DNA barcoding research in Bulgaria.

On 27 September 2023, during a specialised symposium on DNA barcoding at the Bulgarian Academy of Sciences, the Bulgarian Barcode of Life (BgBOL), a Bulgarian DNA barcoding consortium, was founded.

Logo of the Bulgarian Barcode of Life (BgBOL), a Bulgarian DNA barcoding consortium and the latest national node to join the International Barcode of Life Consortium (iBOL).

By becoming the latest national node to join the International Barcode of Life Consortium (iBOL), the main task before BgBOL will be to coordinate, support, and promote DNA barcoding research in Bulgaria, with a primary focus on the study and preservation of the country’s biodiversity.

“The Bulgarian Barcode of Life opens up new horizons and opportunities to study and understand the biodiversity in Bulgaria,”
says Dr Georgi Bonchev, Institute of Plant Physiology and Genetics at the Bulgarian Academy of Sciences (BAS).

DNA barcoding is a method to identify individual organisms based on nucleotide sequences captured from short, predefined and standardised segments of DNA.

Dr Georgi Bonchev explains the DNA barcoding method at the specialised symposium held on 27 September 2023 at the Bulgarian Academy of Sciences.
*Photo by the Bulgarian Academy of Sciences.*

The formation of the BgBOL consortium is expected to strengthen the network of collaborations, ultimately contributing to the broader dissemination and popularisation of DNA barcoding research in the region.BgBOL was created by seven academic institutions: Institute of Plant Physiology and Genetics (BAS), Institute of Biodiversity and Ecosystem Research, National Museum of Natural History (BAS), Sofia University “St. Kliment Ohridski”, AgroBioInstitute (Agricultural Academy), University of Forestry, and Pensoft in its role of a scientific publisher and tech innovator well-known in the field of biodiversity science.

Prof. Lyubomir Penev joined the symposium with a talk on the publication, dissemination and management of DNA barcoding data. His presentation also touched on the relevant biodiversity data workflows and tools currently in development at Pensoft with the support of the Horizon 2020-funded project BiCIKL.
*Photo by the Bulgarian Academy of Sciences*.

As part of the event, Pensoft’s founder and CEO Prof. Lyubomir Penev led a discussion on the publication, dissemination and management of DNA barcoding data. His presentation also touched on the relevant biodiversity data workflows and tools currently in development at Pensoft with the support of the Horizon 2020-funded project BiCIKL (abbreviation for Biodiversity Community Integrated Knowledge Library).

“I’d like to congratulate everyone involved in the establishment of the Bulgarian Barcode of Life! This is a huge step forward in advancing DNA barcoding research in Bulgaria and, ultimately, the preservation of the country’s amazing biodiversity,”
comments Prof. Lyubomir Penev.

Visit the BgBOL website and follow the network on LinkedIn and Facebook, where you might also want to join the BgBOL Facebook group!

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About the International Barcode of Life:

The International Barcode of Life Consortium is a research alliance undertaking the largest global biodiversity science initiative: create a digital identification system for life that is accessible to everyone.

iBOL is working to establish an Earth observation system that will discover species, reveal their interactions, and establish biodiversity baselines. The consortium is tracking ecosystems across the planet and exploring symbiomes – the distinct fungal, plant, and animal species associated with host organisms. Our goal is to complete this research and establish baseline data for science and society’s benefit.