New Study Outlines How Countries Can Build Effective DNA Barcoding Networks

New research in Metabarcoding and Metagenomics provides a blueprint for building a robust, globally adaptable network of DNA barcoding practitioners. 

Aligning with the International Day for Biological Diversity‘s theme of “Acting locally for global impact,” researchers have released a comprehensive blueprint for building a robust, globally adaptable network of DNA barcoding practitioners. 

Heat map illustrating the heterogeneity in the number of available DNA barcode records with species assignment.
Heat map illustrating the heterogeneity in the number of available DNA barcode records with species assignment. Data were retrieved from the BOLD Systems data portal in November 2025. Credit to Kaitetzidou et al., 2026.

DNA barcoding has become a cornerstone for modern species identification and biodiversity monitoring, proving vital for applications ranging from ecological research to conservation and environmental policy. Since its inception, the DNA barcoding community worldwide has been convening under the aegis of the International Barcode of Life (iBOL), which provides necessary global coordination. To implement barcoding at scale, build local capacity, and translate scientific advances into actionable practice, iBOL national nodes have been established since the 2010s

Published in the open-access journal Metabarcoding and Metagenomics, the article “Empowering national capacity for a DNA-based approach to species identification and biodiversity monitoring” by E. Kaitetzidou et al. provides strategic advice on establishing iBOL national nodes. 

Drawing on a survey and workshop conducted under the Horizon Europe Biodiversity Genomics Europe project, this research synthesises experiences from 20 countries, including 17 in Europe, and examines how national nodes are initiated, governed, and sustained. Common challenges included defining scope, securing sustainable funding, harmonising methodologies, and engaging stakeholders. For example, a particularly striking finding was how rarely node coordinators sought guidance from established networks before setting up their own.

The decision-making process and steps taken were almost entirely based on intuition and the experience from other within-country activities, as well as their perceptions of the activities of other nations’ barcoding nodes,

the researchers note. 
Countries are highlighted whose representatives of a national initiative responded to the questionnaire (yellow), attended the workshop (green) or both (yellow–green stripes).
Countries are highlighted whose representatives of a national initiative responded to the questionnaire (yellow), attended the workshop (green) or both (yellow–green stripes). Peru and South Africa are not displayed on the map; however, representatives of their national nodes took part in the survey and the workshop, respectively. Credit to Kaitetzidou et al., 2026.

Central to the paper are ten practical recommendations to ensure the establishment and long-term success of national DNA barcoding nodes. The authors emphasise several key priorities, primarily the construction of comprehensive DNA barcode reference libraries and the critical need to align scientific activities with practical biomonitoring requirements.

Furthermore, they strongly advocate for promoting FAIR (Findable, Accessible, Interoperable, and Reusable) and CARE data principles, alongside implementing focused strategies for capacity building, methodological standardisation, communication, and diverse stakeholder engagement.

Stronger national infrastructures will enhance Europe’s capacity for DNA-based biodiversity monitoring and support metabarcoding and metagenomic research. Building on milestones such as the establishment of iBOL Europe in 2022, these local efforts add up to real progress on species discovery, conservation, and environmental management worldwide. 

This paper has been published with the support of the Biodiversity Genomics Europe project, funded through Horizon Europe, the UK Research & Innovation Fund and the Swiss Confederation (https://biodiversitygenomics.eu/). 

Original source:

Kaitetzidou E, Gadawski P, Goodall-Copestake WP, Dankova G, Gkagkavouzis K, Holak S, Rewicz T, Bącela-Spychalska K, Mamos T, Fantoni K, Jabłońska A, Tończyk G, Trębicki Ł, Aravanopoulos FA, Bruschini C, Bonchev G, Dagher Kharrat MB, Čiampor F, Costa FO, Dapporto L, Ekrem T, Ferreira S, Geiger M, Hausmann A, Hebert PDN, Kalamujić Stroil B, Kamenova S, Kautmanova I, Keskin E, Kučinić M, Lipinskaya T, Mutanen M, Papakostas S, Price B, Ramírez R, Rougerie R, Rulik B, Szucsich N, Van Der Bank M, Triantafyllidis A, Hollingsworth PM, Grabowski M (2026) Empowering national capacity for DNA-based approach to species identification and biodiversity monitoring. Metabarcoding and Metagenomics 10: e183268. https://doi.org/10.3897/mbmg.10.183268

5000 Students run ‘bee hotels’ across Canada – DNA reveals who’s checking in

Students at the forefront of conservation or how community science helps gather data on cavity-nesting bees and wasps, enhancing our understanding of ecosystem interactions.

Can students be the front lines of conservation? A new Canada-wide study, published in Metabarcoding and Metagenomics, suggests they can. The efforts of some 5000 students produced data detailed enough to reveal complex ecological networks hidden inside a small PVC and cardboard tube home.

A trap nest installed at a Bees@Schools community science location. Multiple tubes nested in are visible.
A trap nest installed at a Bees@Schools community science location. Multiple tubes nested in are visible. Photo credit to Sage Handler.

They invited schools to volunteer across Canada to install standardised ‘trap nests’ – simple PVC pipe and cardboard tube homes that mimic natural cavities where bees and wasps build nests.

Cavity-nesting bees and wasps play key roles in pollination and pest control, yet their distributions and feeding relationships are often poorly known because they can be small, secretive and difficult to observe directly.

As part of the Bees@Schools community science program, researchers Sage Handler (University of Guelph), Nigel Raine (University of Guelph), and Dirk Steinke (University of Guelph) aimed to address this.

Nesting tubes gathered from one trap nest, ready to be cut open and processed.
Nesting tubes gathered from one trap nest, ready to be cut open and processed.
Photo credit to Sage Handler.

Instead of relying only on traditional identification under a microscope, the researchers used DNA metabarcoding – a method that reads DNA from mixed samples and can detect many species at once.

This allowed the team to identify not only which bee or wasp species built each nest, but also which plant pollen or insect prey were brought back as food. The result was a rich, detailed view of both where cavity-nesting bee and wasp species live and how they interact with plants and other insects.

A key outcome of the study was the creation of tripartite networks: maps linking (1) the nesting bee or wasp, (2) its food (pollen or insect prey) and (3) parasites. This kind of network is extremely difficult to build through observation alone, but trap nests can act like tiny ecological time capsules.

Every brood cell contains biological traces and metabarcoding can recover them. Students weren’t just collecting insects, they were collecting entire ecological interaction datasets: the raw material needed to build food-web maps across a whole country!

A lot of people want to contribute to conservation or learn more about biodiversity, but don’t know how. This shows that a small, practical action, like hosting a trap nest, can contribute real data that researchers can use. Community and citizen science is becoming more common, so keep an eye out for research happening in your neighbourhood.

says Handler, the lead author of the study

Original resource:

Handler S, Coveny K, Braukmann TWA, Raine NE, Steinke D (2026) Welcome to Hotel Hymenoptera: monitoring cavity-nesting bee and wasp distribution and their trophic interactions using community science and metabarcoding. Metabarcoding and Metagenomics 10: e139674. https://doi.org/10.3897/mbmg.10.139674

Newly established Bulgarian Barcode of Life to support biodiversity conservation in the country

As the latest national node to join the International Barcode of Life Consortium (iBOL), its main task is to coordinate, support, and promote DNA barcoding research in Bulgaria.

On 27 September 2023, during a specialised symposium on DNA barcoding at the Bulgarian Academy of Sciences, the Bulgarian Barcode of Life (BgBOL), a Bulgarian DNA barcoding consortium, was founded. 

Logo of the Bulgarian Barcode of Life (BgBOL), a Bulgarian DNA barcoding consortium and the latest national node to join the International Barcode of Life Consortium (iBOL).

By becoming the latest national node to join the International Barcode of Life Consortium (iBOL), the main task before BgBOL will be to coordinate, support, and promote DNA barcoding research in Bulgaria, with a primary focus on the study and preservation of the country’s biodiversity.

“The Bulgarian Barcode of Life opens up new horizons and opportunities to study and understand the biodiversity in Bulgaria,”

says Dr Georgi Bonchev, Institute of Plant Physiology and Genetics at the Bulgarian Academy of Sciences (BAS).

DNA barcoding is a method to identify individual organisms based on nucleotide sequences captured from short, predefined and standardised segments of DNA.

Dr Georgi Bonchev explains the DNA barcoding method at the specialised symposium held on 27 September 2023 at the Bulgarian Academy of Sciences. 
Photo by the Bulgarian Academy of Sciences.

The formation of the BgBOL consortium is expected to strengthen the network of collaborations, ultimately contributing to the broader dissemination and popularisation of DNA barcoding research in the region.BgBOL was created by seven academic institutions: Institute of Plant Physiology and Genetics (BAS), Institute of Biodiversity and Ecosystem Research, National Museum of Natural History (BAS), Sofia University “St. Kliment Ohridski”, AgroBioInstitute (Agricultural Academy), University of Forestry, and Pensoft in its role of a scientific publisher and tech innovator well-known in the field of biodiversity science.

Prof. Lyubomir Penev joined the symposium with a talk on the publication, dissemination and management of DNA barcoding data. His presentation also touched on the relevant biodiversity data workflows and tools currently in development at Pensoft with the support of the Horizon 2020-funded project BiCIKL.
Photo by the Bulgarian Academy of Sciences.

As part of the event, Pensoft’s founder and CEO Prof. Lyubomir Penev led a discussion on the publication, dissemination and management of DNA barcoding data. His presentation also touched on the relevant biodiversity data workflows and tools currently in development at Pensoft with the support of the Horizon 2020-funded project BiCIKL (abbreviation for Biodiversity Community Integrated Knowledge Library).

“I’d like to congratulate everyone involved in the establishment of the Bulgarian Barcode of Life! This is a huge step forward in advancing DNA barcoding research in Bulgaria and, ultimately, the preservation of the country’s amazing biodiversity,”

comments Prof. Lyubomir Penev.
Visit the BgBOL website and follow the network on LinkedIn and Facebook, where you might also want to join the BgBOL Facebook group!

***

About the International Barcode of Life:

The International Barcode of Life Consortium is a research alliance undertaking the largest global biodiversity science initiative: create a digital identification system for life that is accessible to everyone.

iBOL is working to establish an Earth observation system that will discover species, reveal their interactions, and establish biodiversity baselines. The consortium is tracking ecosystems across the planet and exploring symbiomes – the distinct fungal, plant, and animal species associated with host organisms. Our goal is to complete this research and establish baseline data for science and society’s benefit.