A mystery kept in London led to the discovery of a new Ecuadorian frog

Pristimantis milpe is an abundant and widely distributed species that had been confused with another species for over a century.

Scientists from the Pontificia Universidad Católica del Ecuador officially described Pristimantis milpe, an abundant and widely distributed species that had been confused with another species for over a century. To solve the mystery, researchers reviewed specimens collected in Ecuador during the 19th century and preserved at the Natural History Museum in London.

Reproductive pair (amplexus) of the Milpe Robber Frog photographed at night on a leaf in the tropical forest of the Ecuadorian Chocó. Photo: Santiago R. Ron.

The finding is exceptional because, unlike many recently described species – which are usually rare or restricted to small geographic areas – Pristimantis milpe is a common frog, widely distributed, and easy to detect by its nocturnal call. The species inhabits the Chocó forests of western Ecuador and southern Colombia, in a range that exceeds 67,000 square kilometers, from sea level up to 1,200 meters in altitude.

Amphibiologist Santiago Ron. The leading author of the study.

The scientific description was published in the international journal ZooKeys, which specialises in research on biodiversity and taxonomy. The identification of Pristimantis milpe was made possible thanks to a review of the historical amphibian collections preserved at the Natural History Museum in London (NHM).

Facade of the Natural History Museum in London, where the key specimens that clarified the taxonomic mess are kept. Photo: Santiago R. Ron.

To resolve a taxonomic confusion that lasted for more than a century, Santiago Ron, a researcher at PUCE and lead author of the study, travelled to England to examine the original specimens – known as “types” – that were collected in Ecuador during the 19th century and preserved ever since.

Santiago Ron reviewing jars with historical specimens preserved in the collections of the Natural History Museum in London. Specimens were collected in the 19th century in Ecuador and preserved in London.

The analysis allowed these historical specimens to be compared with current populations of frogs from the Ecuadorian Chocó. The researchers determined that the specimens had been erroneously associated with another species. This confusion caused a species that had been present and observed for decades to remain without a scientific name of its own.

“That such a common species has gone unnoticed under the wrong name for decades highlights the importance of correctly connecting scientific names with living populations in nature.”

Jhael Ortega, a PhD student at Virginia Tech and one of the study authors

The Milpe Robber Frog (Cutín de Milpe) is a species with nocturnal habits. Males usually call from the leaves of shrubs and bromeliads located several meters high within the forest. Their vocalisation is characterised by a loud and distinct sound, similar to a “click.”

One of its most striking features is the dimorphism between females and males. Females present a distinctive color pattern on the groin and thighs, with orange or light blue tones accompanied by black spots. Males, on the other hand, have more uniform shades.

The species is found in the forests of the Ecuadorian Chocó and southern Colombia, one of the most important regions for biodiversity conservation.

The Ecuadorian Chocó forest is home to more than 250 species of amphibians, including the Milpe Robber Frog, Pristimantis milpe. Photo: Santiago R. Ron.

The study demonstrates the scientific value of natural history collections. Specimens preserved for centuries allow current researchers to review classifications, compare characteristics, and answer questions that remained open for generations.

In this case, the analysis of specimens collected in the 19th century, combined with the study of current populations, allowed Pristimantis milpe to be formally recognised as a distinct species.

The work also shows that biodiversity still holds surprises even in abundant and well-known species, and that scientific research requires connecting historical knowledge with the tools and observations available today.

Original source

Ron SR, Paredes-Aguirre P, Paucar DA, López Y, Apunte K, Ortega JA (2026) Long overdue: a new, widespread, species of Pristimantis (Anura, Strabomantidae) from the Chocó region and the synonymy of Pristimantis subsigillatus. ZooKeys 1286: 121-152. https://doi.org/10.3897/zookeys.1286.194383


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A new “groins of fire” frog, from the Peruvian Amazon

“When we found this new species in the Amazon lowlands of central Peru, we were quite surprised and kind of speechless.”

Guest blog post by Germán Chávez

As a South American herpetologist, it is inevitable to be absolutely buzzed every time I hear “Germán, you have to go to the Amazon jungle”. Going to the Amazon forest in Peru is perhaps the most joyful way to do your work. The chances to find so many frogs, lizards, snakes, turtles, and even caimans are really high, so one can’t help but get excited.

The Agua Blanca forest. Photo by Germán Chávez

The thing is, to someone like me who focuses their work on describing new species, the expectations shouldn’t be that high. The Amazon has always been a place full of mysteries, so many explorers, seduced by its enigmatic atmosphere, have gone deeper and deeper into the Amazonia. This has resulted in the description of so many species and very few unexplored places left.

So, when Wilmar Aznaran and I found this new species in the Amazon lowlands of central Peru, a well-visited area, we were quite surprised and kind of speechless. I have to confess that my reaction was “Bloody hell!” Externally, the frog is clearly different from any other similar species, and that was evident for us at the very moment we caught it. Indeed, the first option for the title of our new paper in Evolutionary Systematics was “Expect the unexpected: a new treefrog from the Amazon lowlands of Peru.” We could not believe that a medium-sized arboreal frog had passed in front of other researchers’ eyes, and remained unseen.

Scinax pyroinguinis. Photo by Germán Chávez

Soon we found out that it is not a common species in the area: after catching two individuals, we were unable to find more. Not ready to give up, we went once more time to that site a few months later and our efforts to find it were unsuccessful, so we suggest it is not a common frog.

At that point, we knew that we had a new species on hands, but describing it with only two specimens was challenging. Luis A. García-Ayachi went to the area and his efforts were also unsuccessful. That is when Alessandro Catenazzi joined us, so we decided to add an integrative approach to our work, basing our research on morphological and genetic differences. I can only say thanks to all our co-authors: from then on, everything started to work out.

Scinax pyroinguinis. Photo by Germán Chávez

We noticed there were wildfires in the area, are a serious threat to the frog’s habitat. So it is really curious that the orange pattern on the groins, thighs and shanks of the new species, resembles flames, like those threatening its habitat. No better name for our frog than Scinax pyroinguinis, which literally means “groins of fire”.

We hope that this discovery encourages people  and institutions to protect these remnant forests in central Peru, because they may yet harbour unknown species. If these forests disappear, we will probably lose a diversity that we do not even know now yet, and may never will. It is sort of a race against deforestation and habitat loss, but this doesn’t mean there’s nothing we can do. Research like ours is really important to help put the focus on this place, at least in the short term, and try to attract people to join forces in the conservation of Scinax pyroinguinis and its habitat.

Research article:

Chávez G, Aznaran W, García-Ayachi LA, Catenazzi A (2023) Rising from the ashes: A new treefrog (Anura, Hylidae, Scinax) from a wildfire-threatened area in the Amazon lowlands of central Peru. Evolutionary Systematics 7(1): 183-194. https://doi.org/10.3897/evolsyst.7.102425

New puddle frog species leaps into the spotlight from an unexplored mountain, Ethiopia

Two females and eggs of the newly described species (Phrynobatrachus bibita). Photo by S. Goutte and J. Reyes-Velasco.

A new species of puddle frog has just been discovered by NYU Abu Dhabi researchers at the unexplored and isolated Bibita Mountain in southwestern Ethiopia. The research team named the new species Phrynobatrachus bibita sp. nov., or Bibita Mountain dwarf puddle frog, inspired by its home.

In summer 2018, NYU Abu Dhabi Postdoctoral Associates Sandra Goutte and Jacobo Reyes-Velasco explored an isolated mountain in southwestern Ethiopia where some of the last primary forest of the country remains. Bibita Mountain was under the radars of the team for several years due to its isolation and because no other zoologist had ever explored it before.

“Untouched, isolated, and unexplored: it had all the elements to spike our interest,” says Dr. Reyes-Velasco, who initiated the exploration of the mountain. “We tried to reach Bibita in a previous expedition in 2016 without success. Last summer, we used a different route that brought us to higher elevation,” he added.

Female (Phrynobatrachus bibita) next to egg clutches. Photo by S. Goutte and J. Reyes-Velasco.

Their paper, published in ZooKeys journal, reports that the new, tiny frog (17 mm for males and 20 mm for females) is unique among Ethiopian puddle frogs. Among other morphological features, a slender body with long legs, elongated fingers and toes, and a golden coloration, set this frog apart from its closest relatives.

“When we looked at the frogs, it was obvious that we had found a new species, they look so different from any Ethiopian species we had ever seen before!” explains Dr. Goutte.

Back in NYU Abu Dhabi, the research team sequenced tissue samples from the new species and discovered that Phrynobatrachus bibita sp. nov. is genetically different from any frog species in the region.

“The discovery of such a genetically distinct species in only a couple of days in this mountain is the perfect demonstration of how important it is to assess the biodiversity of this type of places. The Bibita Mountain probably has many more unknown species that await our discovery; it is essential for biologists to discover them in order to protect them and their habitat properly,” explains NYU Abu Dhabi Program Head of Biology and the paper’s lead researcher Stéphane Boissinot, who has been working on Ethiopian frogs since 2010.

/Original text by New York University Abu Dhabi, UAE./

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Original source:

Goutte S, Reyes-Velasco J, Boissinot S (2018) A new species of puddle frog from an unexplored mountain in southwestern Ethiopia (Anura, Phrynobatrachidae, Phrynobatrachus). ZooKeys 824: 53–70. https://doi.org/10.3897/zookeys.824.31570

New species of frog from the Neotropics carries its heart on its skin

In the Neotropics, there is a whole group of so-called glassfrogs that amaze with their transparent skin covering their bellies and showing their organs underneath. A recently discovered new species from Amazonian Ecuador, however, goes a step further to fully expose its heart thanks to the transparent skin stretching all over its chest as well as tummy.

The new amphibian is described by a team of scientists led by Dr. Juan M. Guayasamin, Universidad San Francisco de Quito, Ecuador, in the open access journal ZooKeys.

It can also be distinguished by the relatively large dark green spots at the back of its head and the foremost part of the body. Additionally, the species has a characteristic long call.

The new frog is named Hyalinobatrachium yaku, where the species name (yaku) translates to ‘water’ in the local language Kichwa. Water and, more specifically, slow-flowing streams are crucial for the reproduction of all known glassfrogs.

The reproductive behaviour is also quite unusual in this species. Males are often reported to call from the underside of leaves and look after the egg clutches.

Having identified individuals of the new species at three localities, the researchers note some behavioural differences between the populations. Two of them, spotted in the riverine vegetation of an intact forest in Kallana, have been calling from the underside of leaves a few metres above slow-flowing, relatively narrow and shallow streams. Another frog of the species has been observed in an area covered by secondary forests in the Ecuadorian village of Ahuano. Similarly, the amphibian was found on the underside of a leaf one metre above a slow-flowing, narrow and shallow stream.

oo_135330However, at the third locality – a disturbed secondary forest in San José de Payamino – the studied frogs have been perching on leaves of small shrubs, ferns, and grasses some 30 to 150 cm above the ground. Surprisingly, each of them has been at a distance greater than 30 metres from the nearest stream.

The researchers note that, given the geographic distance of approximately 110 km between the localities where the new species has been found, it is likely that the new species has a broader distribution, including areas in neighbouring Peru.

The uncertainty about its distributional range comes from a number of reasons. Firstly, the species’ tiny size of about 2 cm makes it tough to spot from underneath the leaves. Then, even if specimens of the species have been previously collected, they would be almost impossible to identify from museum collection, as many of the characteristic traits, such as the dark green marks, are getting lost after preservation. This is why the conservation status of the species has been listed as Data Deficient, according to the IUCN Red List criteria.

Nevertheless, the scientists identify the major threats to the species, including oil extraction in the region and the related water pollution, road development, habitat degradation and isolation.

“Glassfrogs presumably require continuous tracts of forest to interact with nearby populations, and roads potentially act as barriers to dispersal for transient individuals,” explain the authors.

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Original source:

Guayasamin JM, Cisneros-Heredia DF, Maynard RJ, Lynch RL, Culebras J, Hamilton PS (2017) A marvelous new glassfrog (Centrolenidae, Hyalinobatrachium) from Amazonian Ecuador. ZooKeys 673: 1-20. https://doi.org/10.3897/zookeys.673.12108