From an Herbarium Cabinet to the Lava Fields of Isabela: Restoring One of the Galápagos’ Rarest Plants

Or how decades of curiosity, teamwork and persistence helped give one of the Galápagos’ rarest plants a second chance.

Guest blog post by Patricia Jaramillo Díaz

When I arrived in the Galápagos in 1996, I never imagined that one of the most meaningful conservation projects of my career would begin inside an herbarium cabinet. At the Charles Darwin Research Station, I spent many hours working with herbarium collections assembled over more than a century by botanists who had explored the archipelago. Each specimen preserved a moment in time – a record of where a species had lived and the landscapes it once inhabited. Together, they formed a living archive of the Galápagos flora.

Two years later, in 1998, one specimen began to capture my attention. Collected near Caleta Tagus on northern Isabela Island in 1962, it belonged to Galvezia leucantha subsp. leucantha, a small shrub found nowhere else on Earth.

Every time I opened that cabinet, I found myself asking the same question: Does this plant still survive? 

Herbarium of Galvezia leucantha subsp. leucantha
Herbarium specimen of Galvezia leucantha subsp. leucantha, collected near Caleta Tagus in 1962. More than six decades later, this historical record helped inspire and guide the restoration of one of the Galápagos’ rarest endemic plants. Courtesy of Patricia Jaramillo Díaz, CDS Herbarium.

At first, it was simply curiosity. Over the years, however, that question grew into something much larger. It inspired field expeditions, nursery experiments, ecological restoration and scientific research spanning almost three decades.

Historical herbarium records and botanical surveys led us to Playa Tortuga Negra, on the remote northern coast of Isabela Island, where the species had last been documented. In 2017, the Galápagos Verde 2050 (GV2050) restoration program began working to prevent the disappearance of this critically endangered population.

Reaching the site was never easy. After travelling by boat along the rugged coastline, we crossed extensive young lava flows under the equatorial sun, carrying equipment and supplies. When we finally located the plants, our excitement quickly gave way to concern. Only a handful of wild individuals remained, some growing from narrow cracks in the lava. Their resilience was extraordinary, but so was their vulnerability.

Standing beside those plants, it became clear that we were looking at much more than a rare shrub. These were the last representatives of a unique evolutionary lineage found nowhere else on Earth. Losing them would mean losing an irreplaceable part of the Galápagos’ botanical heritage.

Our question changed: instead of asking whether Galvezia leucantha still survived, we began asking how we could help ensure that it would continue to survive.

That question became the foundation of a long-term restoration program developed through collaboration among the Charles Darwin Foundation, the Galápagos National Park Directorate, researchers, park rangers, field assistants, students and volunteers.

Researchers collecting and preparing alvezia leucantha subsp. leucantha specimens for the CDS Herbarium at Isabela Island
Researchers and a Galápagos National Park ranger collecting and preparing Galvezia leucantha subsp. leucantha specimens for the CDS Herbarium at Playa Tortuga Negra, Isabela Island, during a collaborative field expedition across several islands of the Galápagos Archipelago in 2012. Photo: Rubén Heleno.

Before restoring the species, however, we first had to learn how to propagate it. Very little information existed. There were no established protocols or published studies describing how to cultivate the species. Germination was inconsistent, seedlings were delicate and survival varied depending on growing conditions.

Through observation, experimentation and patience, we gradually refined propagation techniques, tested different substrates and identified conditions that improved germination and seedling development. The nursery became a living laboratory where research and practical conservation came together. Every healthy seedling represented months of work, from collecting seeds in the field to monitoring germination and growth.

Equally important was documenting what we learned. Developing a propagation protocol strengthened our restoration program while providing practical guidance for future conservation projects involving threatened island plants.

Read our complete Propagation Guide here.

Producing healthy seedlings was only the beginning. Returning them to one of the youngest volcanic landscapes in the Galápagos presented an entirely different challenge. Every restoration campaign required months of preparation. Seedlings were gradually acclimated before transport, while strict biosecurity protocols ensured that no invasive organisms accompanied them into one of the world’s most protected ecosystems.

Moving hundreds of young plants across rough seas and unstable lava fields required close coordination between the Charles Darwin Foundation and the Galápagos National Park Directorate. Once on site, every planting location was carefully selected to maximize the seedlings’ chances of survival.

Collection, sorting, disinfection, and laboratory germination trials of Galvezia leucantha subsp. leucantha seeds. Photos: Paúl Mayorga and Patricia Jaramillo Díaz.

Because northern Isabela experiences prolonged dry seasons and shallow volcanic soils retain very little moisture, we also evaluated techniques to improve plant establishment.

During the first restoration campaigns, hydrogel and the Groasis Waterboxx® were tested to reduce water stress during the most vulnerable stage of seedling development. Planting a seedling takes only a few minutes. Helping it become part of a self-sustaining population takes years. That is why restoration never ends when the last plant is placed in the ground.

Watch the restoration work in the Galápagos.

Researchers Anna Calle-Loor and Nicolás Velasco surveying the restoration site at Tagus Cove, Isabela Island, before planting Galvezia leucantha subsp. leucantha. Photo: Carlos Espinoza/FCD.

Every year, our team returned to Playa Tortuga Negra to monitor the restored plants. We recorded their survival, growth, flowering and fruit production, always asking the same question: Could the population eventually recover on its own?

For several years, the answer remained uncertain. Although the restored plants survived, flowered and set seed, the clearest sign of recovery had yet to appear. Then, three years after the first reintroductions, during a monitoring expedition in 2021, everything changed.

While surveying the restoration site, Galápagos National Park rangers and researchers from the Galápagos Verde 2050 program noticed a tiny seedling emerging from a narrow crack in the lava. Only a few centimeters tall, it was easy to overlook. A closer look revealed something extraordinary: it was not one of the seedlings we had planted.

  • Galvezia leucantha in the lava crack.
  • Detailed images of Galvezia leucantha subsp. leucantha

It had germinated naturally from seeds produced by the restored population. After years of restoration efforts, we were witnessing the first evidence that Galvezia leucantha was once again reproducing in the wild. The discovery filled us with excitement, but also with scientific caution. One naturally established seedling was encouraging, yet we needed to know whether it represented the beginning of broader recovery.

Galvezia leucantha subsp. leucantha seedlings. Left: Monitoring and data collection. Right: Seedlings growing under protective mesh. Photos: Carlos Espinoza.

Subsequent monitoring confirmed that it was not an isolated event. By the end of the monitoring period, our team had recorded four naturally established seedlings, all resulting from natural recruitment rather than nursery-grown plants. They demonstrated that restored individuals were flowering, producing viable seeds and giving rise to a new generation without direct human intervention. They also provided the first clear evidence that restoration was rebuilding not only the population, but the ecological processes need for the species to persist over time.

  • Seedling of Galvezia leucantha
  • Flowering Galvezia leucantha.
  • Flowering Galvezia leucantha.

True restoration begins when nature no longer depends on us.

As the project continued, another chapter brought me back to where everything had begun.

The herbarium specimen that inspired my original question once again became central to our work. Historical collections showed that Galvezia leucantha had once occurred near Caleta Tagus, where it had last been collected more than sixty years earlier. Those specimens, together with field observations and ecological assessments, helped identify suitable sites for restoring part of the species’ historical range.

In 2024, the restoration program expanded to Caleta Tagus, helping re-establish the species in part of its historical range for the first time in more than sixty years. Returning plants there felt like completing a circle.

Decades earlier, botanists had carefully preserved specimens documenting a disappearing population. Without knowing it, they had also preserved information that would later help guide its recovery. For me, this reinforced the extraordinary value of herbaria. They are far more than collections of dried plants; they are archives of biodiversity that connect the past with the future, helping us understand species distributions, identify restoration opportunities and guide conservation decisions.

Learn more in the Galvezia leucantha Restoration Plan

The restoration of Galvezia leucantha also taught us that conservation does not end in the field. Sharing what we learn is just as important. In 2025, nearly three decades of experience came together in a scientific paper, a restoration plan and a propagation guide, ensuring that these lessons could support future restoration efforts.

Looking back today, I do not think first about the number of seedlings we planted or the kilometers we walked across lava fields. I think about the people: the park rangers who protected restoration sites, field assistants who carried plants across difficult terrain, nursery staff who cared for thousands of seedlings, researchers who designed experiments, and students and volunteers whose enthusiasm sustained this project over the years.

Although this story is written from my perspective, the recovery of Galvezia leucantha has always been the result of teamwork. When I first opened that herbarium cabinet nearly three decades ago, I could never have imagined where one preserved specimen would lead.

Today, nearly thirty years later, that question finally has an answer. Yes. Galvezia leucantha still survives.

Galapagos fieldwork
Our work continues in the field and laboratory to support the recovery of this species and, we hope, keep sharing good news through 2050. Photos: Patricia Jaramillo Díaz, Paúl Mayorga, and Carlos Espinoza.

More importantly, it is no longer represented only by a few isolated shrubs growing from cracks in lava. Restored plants are flowering, producing seeds and giving rise to a new generation in the wild. The species still faces challenges, but its future is far brighter than it once seemed.

For us, that is the greatest lesson of this journey. Conservation begins with curiosity, advances through science and succeeds through collaboration. Sometimes, a single herbarium specimen is enough to inspire a question.

Sometimes, answering that question takes nearly thirty years, but the journey is worth every step.

Original source:

Jaramillo Díaz P, Charette C, Calle-Loor A, Espinosa-Ortega N, Mayorga P, Zambrano D, Chango R, Velasco N (2026) Advances in the recovery of Galvezia leucantha subsp. leucantha (Plantaginaceae): restoring a critically endangered species on northern Isabela Island, Galápagos. Nature Conservation 64: 111-133. https://doi.org/10.3897/natureconservation.64.177895

New Workflow Helps Ecologists Measure How Individual Animals Use Their Habitat

A new, reproducible R workflow that enables ecologists to standardise the measurement of individual differences in habitat use based on GPS data, demonstrated using data on 13 lapwings, has been published in Individual-Based Ecology.

The ecological niche concept describes the set of environmental conditions a species needs to survive and reproduce, but it has traditionally been applied at the species or population level – even though individual animals are known to vary widely in behavior, diet, and habitat choice.

Individuals belonging to the same species do not necessarily use or respond to their environment in the same way. This variation has been traditionally treated as statistical noise but now we know that it has important implications for the long-term survival of a species.

said Dr. Takola

Despite this, few tools exist to translate modern tracking data and space use patterns into practical, comparable measures of individual habitat specialisation. This new workflow aims to address this gap.

Dr. Elina Takola built an R workflow letting ecologists standardise individual habitat-use differences using GPS data of 13 lapwings.
Heuristic representation of the different niche levels. A) A community can be represented as a set of species in which each occupies a different niche. B) A species can be represented as a set of metapopulations with different niches. C) A population consists of multiple individuals with different individualised niches (the potential niche is shown with transparent dots, and the realised niche is shown with bold dots). Adapted from Takola and Schielzeth (2022).

Working at the individual level, it treats each animal’s personal range of habitat conditions as its own ‘individualised niche’, distinguishing for each individual between the conditions it actually uses (its realised niche) and the conditions available to it but not necessarily used (its potential niche).

These concepts were defined in a previous study by E. Takola and H. Schielzeth. Takola has now taken these concepts a step further, translating them into a practical workflow for studying individual animals in the wild.

To achieve this, it uses combined mixed-effects resource selection functions such as statistical models that estimate both average habitat preferences and how much individuals deviate from that average with hypervolume exploration methods, a way of mapping all the environmental conditions an individual could occupy as a multidimensional space. 

The full worklfow is now published in the open-access, peer-reviewed Individual-based ecology journal.

How the Workflow Works

Graphical abstract
Graphical abstract of the workflow. Generated by Dr. Takola using ChatGPT.

Implemented entirely in R, the workflow draws on tools from ecological niche modeling, behavioral ecology, and spatial ecology. Further, the workflow is divided into three stages: data preparation, analysis, and output generation.

Throughout, it distinguishes between the environmental conditions available to an individual and the conditions it actually uses, allowing researchers to quantify niche breadth or how broad or narrow an individual’s habitat use is; niche overlap or how much individuals’ habitat use overlaps with one another; and repeatability – how consistent an individual’s habitat use is over time. 

The lapwing case study puts this into practice. By using publicly available GPS tracking data from 13 northern lapwings, the study shows how the workflow integrates multiple environmental layers such as earthworm abundance, human presence, pesticides, management, soil variables, and vegetation. 

Why Individual Variation Matters And Future Outlook

Individual organisms differ in genotype, morphology, life strategy, diet, and behavior. Individual-based approaches capture how animals adapt locally and respond to environmental stress in ways population-level averages can obscure.

This has real implications for conservation. Individual-based models, ones that account for energy costs, demographic trends, habitat-selection patterns, and life-history traits, offer a more accurate, mechanistic picture of wild population dynamics, and a better basis for predicting how populations will fare as conditions change. 

Although demonstrated on the northern lapwing, the workflow is designed to be transferable to other mobile species with GPS tracking data, giving ecologists a general-purpose tool for incorporating individual variation into habitat and conservation models.

explains Takola

The data and code underpinning the workflow are available on GitHub

By making individual specialisation measurable and comparable across taxa and datasets, the workflow can help researchers study niche specialisation and population-level heterogeneity across ecological scales, linking individual-level variation back to population- and species-level patterns, and giving conservationists a tool to move beyond population averages toward more targeted conservation methods. 

Original source:

Takola E (2026) The individualized niche in motion: Quantifying individual specialisation with movement data. Individual-based Ecology 2: e203247. https://doi.org/10.3897/ibe.2.203247

Houseflies and Blowflies – Not Bees – Are Among South Africa’s Best Mango Pollinators, But Climate Change Threatens Future Harvests

Study introduces a new way to measure pollinator “effectiveness,” not just presence, revealing that climate change could shrink suitable habitat for key mango pollinators by more than 40% in coming decades

Some of the most important pollinators for South Africa’s mango industry aren’t bees at all – they’re houseflies and blowflies, according to a recently published study in Frontiers of Biogeography by scientists at University College London (UCL).

The study, led by Matthew Phillips Sánchez and Richard Pearson of UCL’s Centre for Biodiversity and Environment Research, set out to find not just which insects visit mango flowers, but which ones actually pollinate them effectively.

Not all visitors are pollinators

Visiting vs. pollinating species of mango in South Africa. Insect visitors of mango in South Africa (61), composed of five orders and 15 families (‘Visitors’). Compiled from global studies describing visitors to mango flowers, which are also present in South Africa with at least 20 occurrence records on GBIF. Thirty-eight of these are pollinators (pollen deposition effectiveness > 0), belonging to two orders and eight families (‘Pollinators’). Credit to Sánchez and Pearson, 2026.

Mango flowers attract a huge range of insect visitors, but many never touch the flower’s reproductive parts and so transfer no pollen at all. To separate the true pollinators from the freeloaders, the researchers used a Pollen Deposition Effectiveness (PDE) metric, which combines how often a species visits mango flowers, how often those visits make contact with the flower’s reproductive organs, and how much pollen is deposited per visit.

illustrative graphic on mango pollination size
Graphic credit to Matthew Phillips Sánchez, 2026.

Applying this method to 61 known mango-visiting insect species found in South Africa, the team identified 38 true pollinators – and found that flies, not bees, were the amongst the more effective pollinators. Blowflies (family Calliphoridae), the housefly (Musca domestica), and a tiny stingless bee species, Liotrigona bottegoi, ranked as the most effective pollinators identified in the study. Larger insects such as honeybees and carpenter bees (Xylocopa) were generally less effective per visit, likely because their bigger bodies make less consistent contact with the small mango flower’s reproductive structures.

Insects from the orders Lepidoptera (butterflies and moths), Coleoptera (beetles) and Hemiptera (true bugs) were found not to contribute meaningfully to pollination, either because they don’t make contact with the flower’s reproductive parts or because there wasn’t enough data to assess them.

A shrinking future for mango pollinators

The researchers combined their pollinator effectiveness data with species distribution models, statistical tools that predict where a species can survive based on climate, to project how pollinator habitat in South Africa’s main mango-growing provinces (Limpopo, Mpumalanga and KwaZulu-Natal) might change under future climate scenarios.

The results point to widespread declines. On average, pollinator species are projected to lose 42% (under a moderate-emissions scenario) to 43% (under a high-emissions scenario) of their currently suitable habitat by 2061-2080 or 66,663 km2 and 73,608 km2 respectively.

Mango pollinator species richness and potential pollination provision. Species richness and potential pollinator provision under present-day climate (left column) and SDM-predicted changes under two climate change scenarios (right columns). Credit to Sánchez and Pearson, 2026.

Flies were projected to be hit hardest, while some bee species were projected to gain ground in parts of the region. The study also found that pollinator communities are likely to undergo substantial species turnover, with many current pollinators potentially shifting toward the country’s south as the climate changes.

Encouragingly, the researchers found that potential pollination service didn’t decline much faster than pollinator numbers overall – a sign of what ecologists call “functional redundancy,” where many species contribute moderate pollination value rather than the community depending on one or two irreplaceable species. This suggests the loss of some species is less likely to cause a sudden pollination collapse, provided enough functionally similar species remain.

The mango industry

mangoes on a tree
Tomy mangoes by alexanruiz via Envato.

Mango is a significant industry in South Africa, valued at more than US$25 million annually and supporting over 13,000 jobs in processing and related trades, in addition to many smallholder and subsistence growers who rely heavily on wild, unmanaged pollinators.

The authors stress that their study is intended primarily as a proof-of-concept for this new modeling approach, rather than a definitive forecast of mango production. The models estimate climatically suitable habitat, not realised populations, and do not account for land use, habitat availability, or the timing of mango flowering relative to pollinator activity.

Practical steps for growers

pile of mangoes
Pile of Fresh Mangoes by elxeneize via Envato.

The researchers highlight several low-cost conservation measures that could help support wild pollinator populations in mango orchards, including reducing pesticide and herbicide use, setting up low-cost “stink stations” to attract pollinating blowflies, planting native flowering plants or allowing wildflowers to grow between orchard rows to provide food and nesting sites for pollinators, and increasing overall plant diversity within orchards.

Original source: 

Sánchez MP, Pearson R (2026) Biogeography of pollination under climate change: integrating pollinator effectiveness into species distribution models for mango in South Africa. Frontiers of Biogeography 19: e180771. https://doi.org/10.21425/fob.19.180771 

Four Pensoft-Published Journals Now Indexed in CABI

Our in-house indexing team works tirelessly to maintain the highest standards and to secure indexing for Pensoft-published journals across a growing range of indexing services. Our team works closely with journal editors and leverages the automated and manual workflows of the ARPHA Publishing Platform to export rich XML metadata, submit content to global databases, and maintain long-term digital preservation.

As of August 2026, these four Pensoft-published, open-access, peer-reviewed journals are now indexed in CABI

Indexing will begin with the latest volume, covering articles published from the start of 2026.

CABI’s full-text repository, as of May 2026, spans over 1,003,300 documents and continues to grow, fully integrated into CABI’s databases – including CAB Abstracts, Global Health, Subject Collections, and Database Subsets. Furthermore, readers with a CABI subscription gain instant, automatic access to all corresponding full-text material.

This marks a step forward in ensuring that research published in these journals reaches an even wider audience of researchers, policymakers, and practitioners worldwide.

Explore the latest issues of Frontiers of BiogeographyPhytologia Balcanica, Scientific Annals of the Danube Delta Institute, and Vegetation Ecology and Diversity – and stay tuned for individual announcements covering each journal in more detail. 

Translation of the Braun-Blanquet scale to percent in TURBOVEG can bias diversity metrics

Guest blog post by Jürgen Dengler, Iwona Dembicz & Łukasz Kozub

This post refers to the article Translation of the Braun-Blanquet scale to percent in TURBOVEG can bias diversity metrics in Vegetation Classification and Survey (https://doi.org/10.3897/VCS.198373)

Visualisation of different ways of back-transforming 7-step Braun-Blanquet scales to percent for numerical analyses. It is evident that for cover values below 3% (0.5 on the log10 scale), the back-translation of TURBOVEG (yellow) yields systematically too high values, while the two mid-point translations partly over- and partly underestimate the true values (Image taken from Dengler et al. 2026, https://doi.org/10.3897/VCS.198373)

The most widespread approach to vegetation sampling is the use of vegetation plots, i.e., defined areas in which all species present are recorded together with their cover. Cover refers to the percentage of the plot area occupied by the superficial parts of a given species. Still, many vegetation ecologists prefer recording cover on ordinal scales, such as variants of the Braun-Blanquet scale, rather than recording it directly as a percentage. Estimating cover always comes with errors, but using an ordinal scale increases this error as it involves a double transformation, first in the field from the fractional cover to a category of the ordinal scale, and then back from the ordinal scale to a metric scale for all kinds of analyses (Dengler and Dembicz 2023Dembicz and Dengler 2025). Typically, the arithmetic or geometric midpoint of the class boundaries of the respective ordinal scale is recommended when back-translating the data. However, the most widely used software for storing and handling vegetation-plot databases, TURBOVEG (Hennekens and Schaminée 2001), has an inbuilt back-translation deviating from this principle (see Figure 1). We thus asked whether and how the mid-point translation and the TURBOVEG translation might bias a set of widely used biodiversity metrics, namely Shannon diversity, Shannon evenness and Simpson diversity.

In our case study using three different datasets covering a wide array of vegetation types, we found that the two mid-point translations had partly significant, partly non-significant, but in all cases small effects on the biodiversity metrics. By contrast, the inbuilt translation of TURBOVEG had severe distorting effects on all three metrics (see Figure 2). The average increases were about 0.7 units for Shannon diversity and 0.2 units for Shannon evenness, which corresponds to an approximately 30% difference in both cases. For Simpson diversity, the increase on average was smaller, with about 0.1 unit, corresponding to roughly 10%, which is due to the fact that the true Simpson diversity values in our datasets were above 0.8 and thus already close to the theoretical maximum of 1.0.

These differences due to the TURBOVEG settings were way higher than differences in biodiversity metrics often reported as statistically significant and ecologically meaningful. This means that using the TURBOVEG default translations can lead to wrong conclusions in studies using datasets that partly have been recorded on the Braun-Blanquet scale and partly directly in percent. For example, in a temporal comparison where a larger fraction of the older plots has been recorded on the Braun-Blanquet scale than in the newer plots, this methodological artifact could erroneously suggest a biodiversity decline. Likewise, spatial biodiversity patterns can be biased when the fraction of plots recorded with variants of the Braun-Blanquet scale varies between different geographic entities (e.g., countries). It is self-evident that the TURBOVEG default translations will also bias any other response variable that relies on species cover, namely other cover-based biodiversity metrics or community-weighted means of functional traits, albeit we did not quantify the effect sizes in these cases.

Effect of using ordinal scales on three biodiversity metrics in three exemplary datasets when using the conventional back-translation to the arithmetic mid-point of class borders compared to the default back-translation from TURBOVEG (yellow) (Image taken from Dengler et al. 2026, https://doi.org/10.3897/VCS.198373)

We thus recommend that researchers using TURBOVEG should not use the default back-translations of this program but set their own back-translations. This is particularly important when receiving data from the two largest vegetation-plot databases in the world, EVA in Europe (Chytrý et al. 2016) and sPlot globally (Bruelheide et al. 2019), as these databases are run under TURBOVEG 3. However, with an adequate export of the requested data, users can overwrite the TURBOVEG default percent values with more proper values that cause less distortion. It is to be hoped that in a future release of TURBOVEG these mistakes rooted in the early days of the program will be corrected.

How Citizen Science and Fieldwork Rediscovered Papilio alexanor in Southern Italy

The rediscovery of Papilio alexanor in southern Italy marks a historic moment for Italian lepidopterology.

Guest blog post by Paolo Mazzei

For decades, butterfly enthusiasts and lepidopterists across Italy have wondered: could the lost southern populations of the Southern Swallowtail still be out there?

Historical records of Papilio alexanor in Southern Italy were sparse and dated, often dismissed as stray individuals blown in by strong winds from Greece or the Balkans. But for many lepidopterists, alexanor represents a true holy grail – a majestic, bright-yellow species that is strictly protected.

Our research didn’t start in a lab, but with a smartphone screen. 

When Citizen Science Meets Expert Curiosity

male Papilio alexanor
Male Papilio alexanor on C. ruber. Picture by Stefano Meraglia.

In May 2023, a marine biologist kayaking along the rugged cliffs of Basilicata uploaded a brief video to iNaturalist. It captured an unmistakable female Papilio alexanor fluttering around a giant fennel (Ferula) stalk.

A year later, a second record appeared on iNaturalist: a pristine, freshly emerged female photographed by a German tourist in Campania. Together, these two sightings hinted that the species might be more than a mere vagrant here.

Papilio alexanor
Papilio alexanor. Picture by Paolo Mazzei.

This is where the power of modern biodiversity platforms like iNaturalist, Observation.org, and the eBMS (European Butterfly Monitoring Scheme) shines: they act as distributed networks of eyes on the ground, catching subtle shifts in nature that a single research team could easily miss. Inspired by these community sightings, our team from the Italian Lepidopterological Association (ALI), in collaboration with the University of Turin, decided it was time to step in.

A 1,400 km Journey into the Wild

Obtaining research permits for a strictly protected species under EU law takes time, ethics, and rigor. With support from the University of Turin, we secured formal authorization from the Italian Ministry of Environment in early 2026.

Driven by passion, our team funded the expedition ourselves, driving over 1,400 km round trip to systematically survey the steep coastal cliffs and dry habitats along the Campania–Basilicata border.

  • Pupa of Papilio alexanor.
  • Papilio alexanor caterpillar
  • Papilio alexanor caterpillars feeding on a plant
  • Papilio alexanor caterpillars of different instars, feeding on seeds of a plant
  • Papilio alexanor on a plant

The thrill of discovery was immediate. Standing at a cliffside turnout, we watched the first bright-yellow male soar up from the ravine. Soon we spotted courting pairs feeding on red valerian (Centranthus ruber) and females in the act of ovipositing.

Encouraged, we started scouting similar spots nearby, and the hunch paid off. About 15 to 20 km away, we found a second active breeding colony

Surprising Ecology: A New Host Plant for Italy

Female Papilio alexanor laying eggs on the flowers of F. glauca. Picture by Luciano Valeri.

Our fieldwork revealed two ecological twists, since published in our paper in Nota Lepidopterologica.

First, the Northern Italian populations rely on host plants such as Ptychotis saxifraga or Opopanax chironium, but the southern caterpillars feed exclusively on Ferula glauca. This host-plant association was previously documented in Greece and the Balkans, but never before in Italy.

Second, the flight begins significantly earlier here than in northern populations, reflecting local adaptation to the semi-arid, thermo-Mediterranean climate.

Protecting the Future: Simple Actions for Big Impact

Finding a “lost” population is cause for celebration, but it also brings responsibility. These isolated coastal colonies remain vulnerable to habitat fragmentation, fire, climate extremes, and road infrastructure development.

Female Papilio  alexanor
Female Papilio alexanor on C. ruber. Picture by Andrea Baruzzi, 2026,

To help Papilio alexanor keep thriving here, effective conservation management should prioritise targeted monitoring, integrating structured transects through schemes like eBMS to track population trends over time; habitat protection, preserving coastal slope vegetation and preventing over-mowing or destruction of Ferula glauca stands during roadside maintenance; and responsible citizen engagement, encouraging nature lovers, hikers, and photographers to submit observations with precise geolocation, while ensuring that delicate breeding sites remain respected and undisturbed. 

This story is a reminder that remarkable biological discoveries are still waiting to be made right under our noses. All it takes is a curious eye, a smartphone upload, and a community of passionate researchers ready to follow the trail.

Original source:

Meraglia S, Baruzzi A, Mazzei P, Valeri L, Zerunian Z (2026) First documented record of a reproducing population of Papilio alexanor Esper, 1800 (Lepidoptera, Papilionidae) in Southern Italy, with ecological notes. Nota Lepidopterologica 49: 157-170. https://doi.org/10.3897/nl.49.206084 

Five New Wasp Species Discovered In Cyprus, Including One Named After Aphrodite

New research expands the knowledge on chalcid wasps in Cyprus, with five new species described from the island. One was named after the Greek goddess Aphrodite.

Scientists have described five new species of parasitoid wasp from Cyprus, more than doubling the number of chalcid wasps known to be found nowhere else on the island. The wasps, all belonging to the family Pteromalidae, are small insects that play an important role as natural pest controllers, yet remain poorly studied across much of Southeastern Europe and the Middle East.

The research, carried out by Evangelos Koutsoukos and Mircea-Dan Mitroiu and published in the open-access journal ZooKeys, was part of a wider project funded by the UK Government’s Darwin Plus initiative. The project, titled “Species richness and biological invasions of Chalcid wasps in Akrotiri Peninsula”, was administered by the Enalia Physis Environmental Research Centre and supervised by Dr Angeliki Martinou, with support from the National and Kapodistrian University of Athens, the University of Leeds, and Alexandru Ioan Cuza University.

Before the start of this study, our knowledge regarding the chalcid wasp fauna of the Akrotiri Sovereign Base Area (SBA) and the rest of the island of Cyprus was extremely limited,” said Koutsoukos.

Chalcid wasps comprise a hyperdiverse group of insects that serve as biological controllers both in natural and manmade ecosystems, and as such it is crucial to enrich our knowledge, towards understanding their true extend of biodiversity, ecology, and their role in the various trophic webs they participate across different ecosystems.

  • Ablaxia makrisi
  • Erythromalus makrisi.

Each of the five new species was named with a story behind it. Two of the species [Ablaxia makrisi and Erythromalus makrisi] were named after Mr. Christodoulos Makris, prominent Cypriot entomologist, for his contributions on the Cypriot biodiversity, and for collecting the holotypes of these two species,” Koutsoukos explained.

Hemitrichus akrotiriensis. Photo credit to Evangelos Koutsoukos.

Hemitrichus akrotiriensis takes its name from the Akrotiri peninsula, described by Koutsoukos as “a protected area and one the greatest biodiversity hotspots in Cyprus, where the holotype was also collected from.” Another species, Ablaxia toxeftrae, was named after Toxeftra beach in the Akamas peninsula, where its holotype was found.

The final species carries perhaps the most evocative name of all:

Janssoniella aphrodite was named after the Greek Ancient Goddess Aphroditi, since the holotype of this species was collected in Yeroskipou area of Paphos, where the Goddess was worshipped during ancient times.

said Koutsoukos
  • Janssoniella aphrodite
  • Janssoniella aphrodite
  • Janssoniella aphrodite

Before this study, only three chalcid wasp species were known to be unique to Cyprus. “Our collecting efforts yielded many more interesting specimens, which might represent new species as well,” said Koutsoukos, “and as such it is evident that these numbers are an underestimation of the true biodiversity of the island.

The outcomes of this project will contribute towards the enrichment of our knowledge of the biodiversity, not only of the island of Cyprus and the U.K. SBAs, but the whole region’s as well,” Koutsoukos said.

Original study:

Koutsoukos E, Mitroiu M-D (2026) Enriching inventories of rare genera: five new species of Pteromalidae (Hymenoptera, Chalcidoidea) from Cyprus. ZooKeys 1286: 1-16. https://doi.org/10.3897/zookeys.1286.196157

TikTok Video Leads to Discovery of Rare Underground Eel in India

Gangaichthys indonepalicus is the first subterranean fish to be recorded from the Gangetic Basin.

Researchers have discovered a new genus and species of underground earthworm eel living beneath the Indo-Gangetic Plain in Bihar, northern India. The small, colourless creature, named Gangaichthys indonepalicus, is the first subterranean fish to be recorded from the Gangetic Basin.

Close-up of Gangaichthys indonepalicus. Image credit: Ishan Agarwal.

The research, published in ZooKeys, was carried out by an international team of scientists from the Thackeray Wildlife Foundation in Mumbai, India; Shivaji University in Kolhapur; the Shanghai Synchrotron Radiation Facility at the Chinese Academy of Sciences; and the Zoological Survey of India in Gopalpur-on-Sea.

The investigation began after a short video clip posted on TikTok in 2024 showed an unusual tiny fish emerging from a water borewell near the border between India and Nepal.

“The unique morphology of the fish in the video, and the fact that there are no known stygobitic fish known from the region, motivated us to track down the species to ascertain its identity.”

The Research Team

Securing physical samples required an intensive fieldwork effort in Bihar, where the scientists manually pumped thousands of litres of water from local handpumps.

“Procuring samples of this unusual fish was challenging. Identifying potential handpumps (borewells) and wells, and obtaining permission from their owners to extract water for the purpose of searching for the fish proved difficult. This became more intensive when we finally began manually pumping water.”

Akshay Khandekar, Thackeray Wildlife Foundation
Live and habitat photos Gangaichthys indonepalicus
Live and habitat photos Gangaichthys indonepalicus: NRC AA 9519, holotype, 40.6 mm SL: Dorso lateral view of: A. Full body; B. Head; C. Bore-well from which holotype and one (damaged) paratype was collected; D. Bore-well from where another damaged specimen was collected. Image credit: Akshay Khandekar et al., 2026.

The first specimen emerged after pumping approximately 2000 litres of water but sustained damage during the process and died immediately. The second and only intact specimen (later designated as the holotype of this species) was found from the same borewell after pumping an additional 500 litres of water.

Gangaichthys indonepalicus in a syringe. Image credit: Ishan Agarwal.

The newly described fish measures roughly four centimetres in length and features physical adaptations suited to life in total darkness, including reduced eyes covered by skin, an unpigmented translucent body, and an apparent absence of ribs.

Advanced micro-CT scanning and genetic analysis confirmed that it represents a completely distinct genus within the earthworm eel family Chaudhuriidae.

“Gangaichthys means fish of the Ganga. The Ganga or Ganges is one of the most important rivers in India, both as a source of fresh water, food and livelihood for millions of people; besides having an important role in the Hindu religion.

This is the first stygobitic fish from the Indo-Gangetic plains and from the Gangetic basin.”

Ishan Agarwal, Thackeray Wildlife Foundation

As for the naming of the species itself, Tejas Thackeray commented:

“As the species occurs on the border between India and Nepal we picked indonepalicus as the specific epithet, which reflects the distribution of the species.”

Gangaichthys indonepalicus. Image credit: Ishan Agarwal.

Agarwal also reflected on the broader context of the find and the challenges of studying subterranean habitats:

“India is an incredibly diverse country, spanning huge gradients in climate and topography and multiple biogeographic regions. However, there is a huge gap in knowledge, both Linnaean and Wallacean shortfalls, with many more species yet to be discovered.

This exciting discovery is in some ways symptomatic of general biodiversity research in India, though sampling subterranean habitats is especially challenging.”

Original source:

Khandekar A, Thackeray T, He Y, Mohapatra A, Agarwal I (2026) Gangaichthys indonepalicus, a new genus and species of miniature stygobitic earthworm eel (Synbranchiformes, Chaudhuriidae) from alluvial aquifers in the Indo-Gangetic Plain, Bihar, northern India. ZooKeys 1289: 185-202. https://doi.org/10.3897/zookeys.1289.188089


For more articles on zoology, visit the ZooKeys website and follow the journal on BlueSky and Facebook.

A mystery kept in London led to the discovery of a new Ecuadorian frog

Pristimantis milpe is an abundant and widely distributed species that had been confused with another species for over a century.

Scientists from the Pontificia Universidad Católica del Ecuador officially described Pristimantis milpe, an abundant and widely distributed species that had been confused with another species for over a century. To solve the mystery, researchers reviewed specimens collected in Ecuador during the 19th century and preserved at the Natural History Museum in London.

Reproductive pair (amplexus) of the Milpe Robber Frog photographed at night on a leaf in the tropical forest of the Ecuadorian Chocó. Photo: Santiago R. Ron.

The finding is exceptional because, unlike many recently described species – which are usually rare or restricted to small geographic areas – Pristimantis milpe is a common frog, widely distributed, and easy to detect by its nocturnal call. The species inhabits the Chocó forests of western Ecuador and southern Colombia, in a range that exceeds 67,000 square kilometers, from sea level up to 1,200 meters in altitude.

Amphibiologist Santiago Ron. The leading author of the study.

The scientific description was published in the international journal ZooKeys, which specialises in research on biodiversity and taxonomy. The identification of Pristimantis milpe was made possible thanks to a review of the historical amphibian collections preserved at the Natural History Museum in London (NHM).

Facade of the Natural History Museum in London, where the key specimens that clarified the taxonomic mess are kept. Photo: Santiago R. Ron.

To resolve a taxonomic confusion that lasted for more than a century, Santiago Ron, a researcher at PUCE and lead author of the study, travelled to England to examine the original specimens – known as “types” – that were collected in Ecuador during the 19th century and preserved ever since.

Santiago Ron reviewing jars with historical specimens preserved in the collections of the Natural History Museum in London. Specimens were collected in the 19th century in Ecuador and preserved in London.

The analysis allowed these historical specimens to be compared with current populations of frogs from the Ecuadorian Chocó. The researchers determined that the specimens had been erroneously associated with another species. This confusion caused a species that had been present and observed for decades to remain without a scientific name of its own.

“That such a common species has gone unnoticed under the wrong name for decades highlights the importance of correctly connecting scientific names with living populations in nature.”

Jhael Ortega, a PhD student at Virginia Tech and one of the study authors

The Milpe Robber Frog (Cutín de Milpe) is a species with nocturnal habits. Males usually call from the leaves of shrubs and bromeliads located several meters high within the forest. Their vocalisation is characterised by a loud and distinct sound, similar to a “click.”

One of its most striking features is the dimorphism between females and males. Females present a distinctive color pattern on the groin and thighs, with orange or light blue tones accompanied by black spots. Males, on the other hand, have more uniform shades.

The species is found in the forests of the Ecuadorian Chocó and southern Colombia, one of the most important regions for biodiversity conservation.

The Ecuadorian Chocó forest is home to more than 250 species of amphibians, including the Milpe Robber Frog, Pristimantis milpe. Photo: Santiago R. Ron.

The study demonstrates the scientific value of natural history collections. Specimens preserved for centuries allow current researchers to review classifications, compare characteristics, and answer questions that remained open for generations.

In this case, the analysis of specimens collected in the 19th century, combined with the study of current populations, allowed Pristimantis milpe to be formally recognised as a distinct species.

The work also shows that biodiversity still holds surprises even in abundant and well-known species, and that scientific research requires connecting historical knowledge with the tools and observations available today.

Original source

Ron SR, Paredes-Aguirre P, Paucar DA, López Y, Apunte K, Ortega JA (2026) Long overdue: a new, widespread, species of Pristimantis (Anura, Strabomantidae) from the Chocó region and the synonymy of Pristimantis subsigillatus. ZooKeys 1286: 121-152. https://doi.org/10.3897/zookeys.1286.194383


For more articles on zoology, visit the ZooKeys website and follow the journal on BlueSky and Facebook.

From Scroll to Science: How an iNaturalist Photo Revealed an Endangered Mussel in a BC Watershed

A photo on iNaturalist sparked a three-year research project that led to the discovery of a new subpopulation of the endangered Rocky Mountain Ridged Mussel in Canada.

In 2023, someone photographed a discarded shell near a boat-towing business in Port Coquitlam and uploaded it to iNaturalist, the online citizen-science forum. That single post set off a three-year scientific search and ended up rewriting the known range of one of Canada’s most endangered freshwater species. 

The shell belonged to the Rocky Mountain Ridged Mussel (Gonidea angulata), a large, distinct bivalve known for its thick-walled, trapezoidal shell and a sharp ridge running from its dorsal margin to its posterior edge.

Rocky Mountain Ridged Mussel, Dominion Road, August 8, 2025. Photo Angie Coulter.

In Canada, it’s assessed as endangered by the Committee on the Status of Endangered Wildlife in Canada (COSEWIC). It’s also recommended for federal listing under the Species at Risk Act. Until now, its known Canadian range was thought to be confined to the south-central Okanagan watershed, with only vague historical records elsewhere in the Kootenays, Vancouver Island, and Cache Creek. 

That’s what made the Port Coquitlam shell so strange. The Fraser River watershed, where it turned up, has no present-day aquatic connection to the Okanagan system. So how did it get there? 

A photo that sparked two years of research

Because the shell surfaced near a boat-towing business, researchers from the BC Ministry of Water, Land and Resource Stewardship and the BC Conservation Foundation couldn’t rule out a simpler explanation: that it had hitched a ride from the Okanagan on a recreational boat trailer. 

  • Researchers at Surrey Bend Regional Park,  Sept 10, 2024. Photo by Kihan Yoon-Henderson.
  • Sampling in Harrison River, September 2025. Photo by Jennifer Heron.
  • Siena Achal and Kihan Yoon-Henderson on field work.
Photo by Jennifer Heron, Pitt River, Dominion Ave., Sept 9, 2024 

But, to prove that the mussel actually belonged in the Fraser watershed the researchers had to find live, animals in their natural habitat.

  • Pitt River, Reichenbach Rd., Sept 16, 2024. Photo by Jennifer Heron.
  • Kihan Yoon-Henderson, Jennifer Heron, Siena Achal, Mike Badry, Lea Gelling and Purnima Govindarajulu at Grant Narrows – Pitt-Addington WMA, Oct 9, 2024. Photo by Kihan Yoon-Henderson.

So began a major field effort. Between 2023 and 2025, the team surveyed 62 sites and logged more than 158 cumulative hours searching the watershed, contending with busy marine traffic, fast-moving and deep water, rising tides, and the seasonal freshet.

They worked the shifting, tidal shoreline using beach walking, wading, and snorkeling surveys. And because the water was frequently clouded by disturbed silt and mud, the researchers had to get creative, constructing a DIY dipping tool by duct-taping a metal mesh deep-fryer basket to a broom handle. This device allowed them to scoop up submerged objects and let the silt wash away, making it easy to confirm the identity of any mussels they retrieved.

The find

The persistence paid off. About 5.4 kilometers upstream from where the original shell was found, the team confirmed a live, subpopulation – 81 specimens in total across the three-year search.

Most were tucked into deep pools that become isolated from the main river channel at low tide, along a stretch of flat mud beach on the west side of the lower Pitt River, near a public trail between Prairie Avenue and Dominion Avenue.

Rocky Mountain Ridged Mussel, Pitt River, Prairie Ave., Sept 18, 2024, Photo by Jennifer Heron.

The discovery, now published in the open-access, peer-reviewed Biodiversity Data Journal, marks a significant range extension for the species into the Fraser River watershed – the first confirmed live population outside the Okanagan.

  • Bert Brink Wildlife Management Area, July 2025. Photo Jennifer Heron.
  • Domion Road, August 8, 2026. Photo Kihan Yoon-Henderson.
  • Under Mission Bridge, July 2025. Photo Jennifer Heron

However, the find doesn’t ease the species’ conservation status. Because this subpopulation is hydrologically isolated from both the Okanagan watershed and the nearest known populations in Washington State’s Chehalis River, the overall conservation concern for the species remains extremely high.

Whether the newly found subpopulation is genetically distinct from other populations – a question with real implications for how it’s managed and protected – is still unanswered; that’s the next piece of research the team is pursuing. 

Nonetheless, this breakthrough stands as a testament to the value of citizen science. The upload of a single photograph to iNaturalist sparked a big field survey, proving that community-based monitoring and public stewardship can directly help protect B.C.’s most vulnerable freshwater species.

Original source:

Heron J, Jessome R, Yoon-Henderson K, Achal S (2026) Range extension and natural history notes on Rocky Mountain Ridged Mussel Gonidea angulata (Lea, 1838) (Mollusca, Unionidae) in Canada. Biodiversity Data Journal 14: e191607. https://doi.org/10.3897/BDJ.14.e191607